Journal: BMC Gastroenterology

Article Title: Elevated CircYthdc2 expression is correlated with aggressive features and poor progression-free survival in hepatocellular carcinoma

doi: 10.1186/s12876-025-04231-0

Figure Lengend Snippet: YTHDC2 methylation and survival analysis in LIHC. A Analysis of YTHDC2 promoter methylation levels in 377 LIHC tissues compared to 50 normal liver tissues using the UALCAN database, showing significant hypomethylation in LIHC tissues. B Analysis of YTHDC2 expression levels in 371 LIHC tissues compared to 50 normal liver tissues using the UALCAN database, showing significant upregulation in LIHC tissues

Article Snippet: Human liver hepatocellular carcinoma cell lines (Bel-7402 and PLC/PRF/5) and normal human liver cell line L02 (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (Gibco) at 37 °C with 5% CO 2 in a humidified incubator.

Techniques: Methylation, Expressing

Journal: BMC Gastroenterology

Article Title: Elevated CircYthdc2 expression is correlated with aggressive features and poor progression-free survival in hepatocellular carcinoma

doi: 10.1186/s12876-025-04231-0

Figure Lengend Snippet: CircYTHDC2 expression analysis across multiple LIHC models. A RT-qPCR analysis of circYTHDC2 expression in 72 paired LIHC tissues and adjacent normal tissues ( P < 0.001). B Serum circYTHDC2 levels measured by RT-qPCR in 72 LIHC patients, 40 cirrhosis patients and 72 healthy controls. C CircYTHDC2 expression levels in LIHC cell lines (Bel-7402 and PLC/PRF/5) compared to normal human liver cell line L02. *** P < 0.001

Article Snippet: Human liver hepatocellular carcinoma cell lines (Bel-7402 and PLC/PRF/5) and normal human liver cell line L02 (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (Gibco) at 37 °C with 5% CO 2 in a humidified incubator.

Techniques: Expressing, Quantitative RT-PCR

Journal: BMC Gastroenterology

Article Title: Elevated CircYthdc2 expression is correlated with aggressive features and poor progression-free survival in hepatocellular carcinoma

doi: 10.1186/s12876-025-04231-0

Figure Lengend Snippet: Diagnostic potential and stability analysis of circYTHDC2. A ROC curve analysis of tissue circYTHDC2 expression for LIHC diagnosis (AUC: 0.846, 95% CI: 0.775–0.914; sensitivity: 82.4%, specificity: 76.8%). B ROC curve analysis of serum circYTHDC2 levels (AUC: 0.788, 95% CI: 0.703–0.873). C RNase R resistance assay in Bel-7402 cells showing maintained circYTHDC2 levels compared to linear YTHDC2 mRNA after RNase R treatment. D RNase R resistance assay in L02 cells demonstrating similar stability characteristics of circYTHDC2

Article Snippet: Human liver hepatocellular carcinoma cell lines (Bel-7402 and PLC/PRF/5) and normal human liver cell line L02 (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (Gibco) at 37 °C with 5% CO 2 in a humidified incubator.

Techniques: Diagnostic Assay, Expressing, Biomarker Discovery

Journal: BMC Gastroenterology

Article Title: Elevated CircYthdc2 expression is correlated with aggressive features and poor progression-free survival in hepatocellular carcinoma

doi: 10.1186/s12876-025-04231-0

Figure Lengend Snippet: Survival analysis based on circYTHDC2 expression levels. A Kaplan-Meier analysis of progression-free survival in LIHC patients stratified by circYTHDC2 expression levels. B Overall survival analysis comparing high and low circYTHDC2 expression groups. Patients were stratified based on median circYTHDC2 expression level (13.41). Risk tables below each curve indicate the number of patients at risk at 0, 10, 20, and 30 months of follow-up

Article Snippet: Human liver hepatocellular carcinoma cell lines (Bel-7402 and PLC/PRF/5) and normal human liver cell line L02 (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (Gibco) at 37 °C with 5% CO 2 in a humidified incubator.

Techniques: Expressing

Journal: BMC Gastroenterology

Article Title: Elevated CircYthdc2 expression is correlated with aggressive features and poor progression-free survival in hepatocellular carcinoma

doi: 10.1186/s12876-025-04231-0

Figure Lengend Snippet: YTHDC2 stabilizes circYTHDC2 via m6A modification. A Pearson correlation analysis was used to assess the correlation between the expression of YTHDC2 and circYTHDC2 in the tumor samples from LIHC patients ( n = 72). B qRT-PCR analysis was conducted to determine the overexpression efficiency of YTHDC2 in Bel-7402 and PLC/PRF/5 cells. C qRT-PCR analysis was used to measure the expression of circYTHDC2 in Bel-7402 and PLC/PRF/5 cells after YTHDC2 overexpression. D The impact of YTHDC2 overexpression on the RNA stability of circYTHDC2 was measured in Bel-7402 and PLC/PRF/5 cells with ActD treatment for indicated time. E RIP assays were used to detect the binding between anti-m6A with circYTHDC2 in Bel-7402 and PLC/PRF/5 cells after YTHDC2 overexpression. F RIP asssays were performed to explore the interaction between circYTHDC2 and YTHDC2 in Bel-7402 and PLC/PRF/5 cells. G RNA-Pull down assays were used to determine the binding between circYTHDC2 and YTHDC2 in Bel-7402 and PLC/PRF/5 cells. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Human liver hepatocellular carcinoma cell lines (Bel-7402 and PLC/PRF/5) and normal human liver cell line L02 (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (Gibco) at 37 °C with 5% CO 2 in a humidified incubator.

Techniques: Modification, Expressing, Quantitative RT-PCR, Over Expression, Binding Assay